An Exogenous Hydrogen Sulphide Donor, NaHS, Inhibits the Nuclear Factor κB Inhibitor Kinase/Nuclear Factor κB Inhibitor/Nuclear Factor-κB Signaling Pathway and Exerts Cardioprotective Effects in a Rat Hemorrhagic Shock Model

Hemorrhagic shock (HS) is a common condition and leading cause of death in trauma patients universally. Severe inflammatory responses during HS finally lead to multiple-organ failure. Hydrogen sulphide (H2S) is increasingly recognized as an important signaling molecule with various protective effects. In the present study, we investigated the antiinflammatory and cardioprotective effects of an exogenous H2S donor, sodium hydrosulfide (NaHS), in an HS rat model. Male Sprague-Dawley rats were randomly divided into the sham-operated, sham-operated treated with NaHS (28 mu mol/kg, intraperitoneally (i.p.)), HS, and HS treated with NaHS (28 mu mol/kg, i.p.) groups. The HS groups were subjected to mimicked HS for I h and then treated with NaHS or left untreated. The rats were then resuscitated with Ringer lactate solution for I h. Myocardial enzymes and inflammatory cytokines were evaluated. Morphologic changes in cardiac tissue and ultrastructural injury were also analyzed. HS resulted in significant hemodynamic deterioration and increased myocardial enzyme and inflammatory cytokine levels. Intraperitoneal administration of NaHS significantly prevented hemodynamic deterioration and decreased the elevation of myocardial enzymes. NaHS also inhibited the nuclear factor kappa B inhibitor kinase (IKK)/nuclear factor kappa B inhibitor (I kappa B)/nuclear factor kappa B (NF-kappa B) signaling pathway. The results suggest that NaHS exerts cardioprotective effects against HS. The protective effects of NaHS may occur via down-regulation of the IKK/I kappa B/NF-kappa B signaling pathway.