Journal
GUT
Volume 47, Issue 4, Pages 481-486Publisher
BRITISH MED JOURNAL PUBL GROUP
DOI: 10.1136/gut.47.4.481
Keywords
leptin; leptin receptor isoforms; immunohistochemistry; gastric mucosa
Categories
Ask authors/readers for more resources
Background-Leptin is an important regulator of food intake and energy expenditure. Initially it was thought to be expressed exclusively in and secreted by adipocytcs. Recently, leptin expression was also noted in other tissues, including rat gastric mucosa. Information on leptin and leptin receptor expression in the human stomach is lacking. Aim-To investigate expression of leptin and its corresponding receptors in human gastric epithelial cells. Methods- Fundic and antral gastric mucosal biopsies, primary cultures of human gastric epithelial cells, and the human gastric cancer cell line AGS were screened for expression of leptin and different leptin receptor isoform mRNA by reverse transcriptase-polymerase chain reaction. Immunohistochemistry was performed for localisation of leptin and leptin receptor proteins in gastric mucosa. Results-mRNA of leptin and its four receptor isoforms (huOB-R, long receptor isoform; huB219.1-3, short receptor isoforms) was detected in gastric mucosal biopsies, cultured human gastric epithelial cells, and gastric cancer cells. Immune-histochemistry demonstrated that chief as well as parietal cells were reactive to leptin and leptin receptors. Conclusions-Leptin and leptin receptors are expressed in human gastric mucosa. These findings suggest a paracrine and/or autocrine effect of leptin on gastric epithelial cell function.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available