Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 33, Issue 8, Pages 1274-1278Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.33.1274
Keywords
lectin; Musca domestica larva; antitumor; apoptosis; mitochondrial path
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Funding
- National High Technology Research and Development Program of China (863 Program) [2007AA10Z319]
- Chinese National Natural Science Foundation [20676103]
- Tianjin Natural Science Foundation [07JCZDJC02900]
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A lectin from Musca domestica larva was purified by affinity chromatography on a glactose-Sepharose 4B column. Musca domestica larva lectin (MLL) inhibited the growth of BEL-7402 cells in a time and concentration-dependent way. The results of Hoechst 33258 staining indicated that MLL induce BEL-7402 cells apoptosis based on the typical apoptotic morphological changes. Subsequently, we found that MLL treatment abrogated glutathione antioxidant system and induced mitochondria to generate reactive oxygen species (ROS) accumulation, resulting in reduction of mitochondrial transmembrane potential. The induction of cell apoptosis was caused by the upregulation of Bax, the downregulation of Bcl-2, the cytochrome c release and the activation of the caspases pathways.
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