Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 32, Issue 3, Pages 399-404Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.32.399
Keywords
plasma nitrite; kinetic parameter; arteriovenous difference; anesthetized rabbit; authentic nitric oxide
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Funding
- Kanazawa Medical University [S2005-10]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18590247]
- MEXT
- Musashino University
- Grants-in-Aid for Scientific Research [18590247] Funding Source: KAKEN
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Despite of the importance of plasma NO2- as an index of endothelial nitric oxide (NO) formation and a substrate for NO production, only a limited kinetic knowledge is available in vivo. To address this issue, we intravenously injected NaNO2 into anesthetized rabbits and quantified changes in arterial and venous plasma NO2- levels. Plasma NO2 levels in arterial blood (956 +/- 220 nM) were slightly, but significantly, higher than those in venous blood (889 +/- 214 nM) under control conditions. Although similar half-lives of the NO2- were observed in arterial and venous plasma, significant arteriovenous differences were observed fit the volume of distribution of the central compartment (0.158 +/- 0.0071/kg in arterial blood and 0.295 +/- 0.0151/kg in venous blood) and the peripheral compartment (0.259 +/- 0.0351/kg in arterial blood and 0.135 +/- 0.0341/kg in venous blood). When NOR1 (anthentic NO) was injected, increases in NO2- levels were greater in arterial plasma, and decreases in blood pressure significantly correlated with arterial NO2- levels only (r=0.90, p<0.01). These results indicate that changes in NO2- are larger and more easily detectable as an index of NO formation in arterial plasma.
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