Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 31, Issue 6, Pages 1092-1095Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.31.1092
Keywords
DNA replication; cell proliferation; adipogenesis; fad24; histone acetyltransferase binding to ORC1 (HBO1)
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- Japan Society for the Promotion of Science (JSPS)
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A novel gene factor for adipocyte differentiation 24 (fad24), promotes adipogenesis by controlling DNA replication early on during a stage referred to as mitotic clonal expansion (MCE). MCE is considered distinct from the proliferation of pre-confluent cells, so we investigated the role of fad24 in the process. First, the expression of fad24 was examined in pre-confluent and post-confluent 3T3-L1 preadipocytes, NIH-3T3 fibroblasts, and C2C12 myoblasts. fad24 was strongly expressed in the pre-confluent cells. The knockdown of fad24 by RNA interference impaired the ability of the pre-confluent cells to proliferate. Moreover, bromodeoxyuridine labeling and chromatin immunoprecipitation experiments indicated that the knockdown inhibited DNA synthesis by preventing the recruitment of histone acetyltransferase binding to ORC1 (HBO1), a component of the pre-replicative complex, to origins. fad24 plays positive roles in the proliferation of pre-confluent cells as well as adipogenesis.
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