Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 31, Issue 12, Pages 2234-2236Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.31.2234
Keywords
Candida albicans; fluconazole; baicalein; synergism; drug resistance
Categories
Funding
- National Natural Science Foundation of China [30630071]
- Key Scientific and Technological Program Project Grant of Shanghai [06XD14027]
- Shanghai Rising-Star Program [08QA14001]
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In vitro interaction of fluconazole and baicalein (BE) was investigated against 30 fluconazole-resistant clinical isolates of Candida albicans. Synergistic activities were determined using the checkerboard microdilution assay based on the fractional inhibitory concentration indices. Organisms were also tested against the 2 drugs singly and in combination using time-kill methods. Both fluconazole and BE showed weak antifungal activity when tested alone. However, the combination of fluconazole and BE showed strong antifungal activity against most of the fluconazole-resistant isolates tested. The findings of time-kill curves confirmed the interaction. Yeast cells grown in the presence of BE exhibited a reduced extrusion of Rhodamine 6G, which indicates the inhibition of efflux pumps by BE. This novel synergism of fluconazole and BE that can overcome drug-resistance in yeast may prove useful in combined treatment of fungal infections.
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