Journal
BIOLOGIA PLANTARUM
Volume 58, Issue 3, Pages 499-506Publisher
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s10535-014-0432-7
Keywords
amino acid sequence; apple; dehydration responsive element; Malus domestica; RT-PCR
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Funding
- National Natural Science Foundation of China [30800602]
- Shanghai PuJiang Program [11PJ1408500]
- PaoGao Program
- Key Project Fund of the Shanghai Municipal Committee of Agriculture [2011-1-8]
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Transcription factors play vital roles in stress signal transduction and gene expression modulation. The sequence analysis shows that MdCBF1 from Malus domestica Borkh. cv. Fuji contained an AP2 core domain of 56 amino acids. By comparison of deduced amino acid sequences of CBF related proteins, we deduced that MdCBF1 is a CBF transcription factor gene which belongs to AP2/EREBP family, DREB-A1 subfamily. Further, we reported that transgenic Arabidopsis thaliana plants expressing the MdCBF1 gene exhibited stronger growth than wild type plants under freezing stress. The analysis of RT-PCR for stress-responsive genes implied that MdCBF1 over-expressing plants had a higher expression of COR15a, RD29A, and RD29B genes than wild type plants. Collectively, our results indicate that MdCBF1 might play an important role in the response of transgenic Arabidopsis plants to freezing stress.
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