Journal
BIOLOGIA PLANTARUM
Volume 58, Issue 1, Pages 89-98Publisher
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s10535-013-0373-6
Keywords
abscisic acid; drought tolerance; low temperature; Nicotiana tabacum; Triticum aestivum
Categories
Funding
- National Transgenic Major Project [2012ZX08002-004]
- Shandong Agriculture and Seed Industry
- China Agriculture Research System [CARS-03-1-08]
- Funding for the Post-doctoral Innovative Projects of Shandong Province [201203024]
- Shandong Agriculture Research System
- National Key Technology RAMP
- D program of China [2011BAD35B03]
- Outstanding Agricultural Talent and Team
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Abscisic acid (ABA) regulates plant responses to various environmental stresses. Oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the biosynthesis of ABA in higher plants. Using a homologous cloning approach, a NCED-like gene (designated as TaNCED1) was isolated from wheat (Triticum aestivum). It contained an open reading frame of 1 848 bp and encodes a peptide of 615 amino acids. Multiple sequence alignments showed that TaNCED1 shared high identity with NCEDs from other plants. Phylogenetic analysis revealed that TaNCED1 was most closely related to a barley HvNCED1 gene. The predicted 3D structure of TaNCED1 showed high similarity with other homologues. Southern blot analysis indicated that TaNCED1 was a single copy in the genome of wheat. TaNCED1 was differentially expressed in various organs and the expression was up-regulated by low temperature, drought, NaCl, and ABA. Heterologous expression of TaNCED1 in tobacco (Nicotiana tabacum) significantly improved its drought tolerance. Under drought treatment, TaNCED1-overexpressing transgenic tobacco plants exhibited higher germination rate, higher relative water content, content of soluble sugars and of ABA when compared with the wild type plants.
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