Sunlight-activated insecticides: historical background and mechanisms of phototoxic activity

Several photosensitizing agents, which are activated by illumination with sunlight or artificial light sources, have been shown to be accumulated in significant amounts by a variety of insects when they are administered in association with suitable baits. The subsequent exposure of such insects to UV/visible light leads to a significant drop in survival. Of the photosensitizers tested so far, xanthenes (e.g. phloxin B) and porphyrins (e.g. haematoporphyrin) appear to be endowed with the highest photoinsecticidal activity. In particular, porphyrins absorb essentially all the UV/visible light wavelengths in the emission spectrum of the sun; hence they are active at very low doses. Thus, 1 h irradiation of Ceratitis capitata, Bactrocera oleae (also known as Dacus oleae) or Stomoxys calcitrans which ingested a few nanomoles of porphyrin per fly with light intensities of the order of 1000 mu E s(-1) m(-2) causes about 100% death in laboratory tests. Present evidence suggests that such photosensitizers act on the membranes of the midgut with consequent feeding inhibition, as well as on the neuromuscnlar sheath. No apparent onset of photoresistance has been observed. The rapid photobleaching of xanthenes and porphyrins when illuminated by visible light, as well as the lack of significant toxicity of such compounds in the dark, minimizes the risk of an important environmental impact of such photoinsecticidal agents. (C) 2000 Elsevier Science Ltd. All rights reserved.