Journal
BIOLOGIA PLANTARUM
Volume 57, Issue 4, Pages 793-796Publisher
ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
DOI: 10.1007/s10535-013-0357-6
Keywords
ABA; mannitol; medicinal plant; micropropagation; St. John's wort
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Funding
- Bulgarian Ministry of Education, Youth, and Science [DNTC BG-SK-01/2012]
- Slovak Research and Development Agency [APVV SK-BG 0012-10]
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The ultrastructure of leaf mesophyll cells of in vitro cultured Hypericum perforatum L. plants regenerated after cryopreservation was studied. Electron microscopy analysis revealed that the chloroplasts in plants pretreated with abscisic acid and regenerated after cryopreservation were round, with increased amount of starch, rather small volume of the thylakoid system, and destroyed envelope. Plants pretreated with 0.3 M mannitol and cooled at rates of 0.1 or 0.3 A degrees C min(-1) possessed chloroplasts with high starch content that resulted in a reduction of a membrane system. However, the pretreatment with 0.3 M mannitol and cooling at a rate of 0.2 A degrees C min(-1) was the best as chloroplast ultrastructure resembled the controls regenerated without cryopreservation.
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