Journal
BIOPHYSICAL JOURNAL
Volume 79, Issue 4, Pages 2199-2208Publisher
BIOPHYSICAL SOCIETY
DOI: 10.1016/S0006-3495(00)76468-X
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Funding
- NINDS NIH HHS [NS24692] Funding Source: Medline
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Genetically encoded reporters for optical measurements of presynaptic activity hold significant promise for measurements of neurotransmission within intact or semi-intact neuronal networks. We have characterized pH-sensitive green fluorescent protein-based sensors (pHluorins) of synaptic vesicle cycling at nerve terminals. pHluorins have a pK similar to 7.1, which make them ideal for tracking synaptic vesicle lumen pH upon cycling through the plasma membrane during action potentials. A theoretical analysis of the expected signals using this approach and guidelines for future reporter development are provided.
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