Journal
BIOLOGIA PLANTARUM
Volume 57, Issue 1, Pages 154-158Publisher
SPRINGER
DOI: 10.1007/s10535-012-0128-9
Keywords
CAD; chlorophyll; 24-epibrassinolide; G6PDH; GST; malondialdehyde; PAL; SKDH; Solanum lycopersicum
Categories
Funding
- National Basic Research Program of China [2009CB119000]
- National Natural Science Foundation of China [31071790]
- National Key Technology R&D Program of China [2011BAD12B04]
Ask authors/readers for more resources
We investigated the role of 24-epibrassinolide (EBR) in the amelioration of phenanthrene (PHE) stress in tomato (Solanum lycopersicum L.). Exposure to PHE (300 mu M) significantly decreased shoot and root length (19 and 16 %, respectively), fresh mass (35 and 43 %, respectively), contents of chlorophyll a (26 %), chlorophyll b (27 %) and carotenoids (18 %) in tomato plants. In addition, PHE increased the malondialdehyde (MDA) content (57 %) and activity of secondary metabolism related enzymes glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PDH), shikimate dehydrogenase (SKDH), phenylalanine ammonia-lyase (PAL) and cinnamyl alcohol dehydrogenase (CAD). The expression levels of GST1, PPO, SKDH, PAL and CAD genes were also induced by PHE. Importantly, EBR (0.1 mu M) alone and in combination with PHE increased the growth, biomass and activity of those enzymes significantly over control and PHE alone, respectively. Consistent with enzymes activities transcript levels of GST1, PPO, SKDH, PAL and CAD were further increased in PHE+EBR over PHE alone. However, MDA content was remarkably decreased in PHE+EBR than PHE alone. Meanwhile, content of phenols, flavonoids and antioxidant activity were increased by PHE and PHE+EBR further increased all those parameters. These observations suggest that EBR regulates secondary metabolism in tomato which might enhance tolerance to PHE.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available