Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 10, Pages 4595-4597Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.10.4595-4597.2000
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The inhibitory activities of known microcins were evaluated against some diarrheagenic Escherichia coli strains. Some antibacterial properties of microcin J25, the most active one, were studied. A rapid two-step purification was performed. The MIC and the minimum bactericidal concentration of J25 against E. coli O157:H7 were 1 and 100 mu g ml(-1), respectively. A 10(4)-CFU ml(-1) contamination by this strain was destroyed in milk and meat extract by 6.25 mu g of J25 ml(-1) and in half-diluted egg yolk by 50 mu g of J25 ml(-1).
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