Journal
BIOPHYSICAL JOURNAL
Volume 79, Issue 4, Pages 1761-1770Publisher
CELL PRESS
DOI: 10.1016/S0006-3495(00)76428-9
Keywords
-
Categories
Funding
- NIGMS NIH HHS [GM59018-01] Funding Source: Medline
Ask authors/readers for more resources
A continuum description for diffusion in a simple model for an inhomogeneous but isotropic media is derived and implemented numerically. The locally averaged density of diffusible marker is input from experiment to define the sample. Then a single additional parameter, the effective diffusion constant, permits the quantitative simulation of diffusive relaxation from any initial condition. Using this simulation, it is possible to model the recovery of a fluorescently tagged protein in the endoplasmic reticulum (ER) after photobleaching a substantial region of a live cell, and fit an effective diffusion constant which is a property both of the geometry of the ER and the marker. Such quantitative measurements permit inferences about the topology and internal organization of this organelle.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available