Journal
BIOLOGIA PLANTARUM
Volume 54, Issue 2, Pages 334-338Publisher
SPRINGER
DOI: 10.1007/s10535-010-0058-3
Keywords
genetic stability; in vitro; medicinal plant; polymerase chain reaction
Categories
Funding
- Indian Council of Agricultural Research (ICAR)
Ask authors/readers for more resources
Rapid micropropagation was achieved in Chlorophytum borivilianum Santapau and Fernandes using shoot base as explants. Multiple shoots were induced on Murashige and Skoog's (MS) medium supplemented with 3.0 mg dm(-3) 6-benzylaminopurine, 0.1 mg dm(-3) 1-naphthaleneacetic acid, 150 mg dm(-3) adenine sulphates and 3 % saccharose. Rooting was readily achieved upon transferring the shoots onto half strength MS medium supplemented with 0.1 mg dm(-3) indolebutyric acid and 2 % saccharose. Micropropagated plantlets were hardened in the greenhouse and successfully established in soil. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of the micropropagated plants. Thirty one arbitrary decamers were used to amplify genomic DNA from in vitro and in vivo plant material to assess the genetic stability. All RAPD profile analysis from micropropagated plants was genetically similar to mother plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available