Journal
BRITISH JOURNAL OF DERMATOLOGY
Volume 143, Issue 4, Pages 728-732Publisher
WILEY
DOI: 10.1046/j.1365-2133.2000.03766.x
Keywords
dermal neutrophil; elafin; interleukin-1 beta; tumour necrosis factor-alpha
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Background Elafin, an elastase inhibitor produced by keratinocytes, is overexpressed in the subcorneal region of skin affected by psoriasis, a major feature of which is epidermal infiltration by neutrophil leucocytes. Objectives We studied the expression of elafin in the epidermis in other skin disorders characterized by dermal neutrophil infiltration and in skin disorders with dermal lymphocyte infiltration. Patients/methods We examined biopsies from the lesional skin of patients with Behcet's syndrome, Sweet's syndrome, pyoderma gangrenosum, cutaneous allergic vasculitis and acute bacterial infection (cellulitis), and from the skin of patients with chronic prurigo, discoid lupus erythematosus and psoriasis. We performed in vitro experiments using cultured keratinocytes treated with mediators such as interleukin (IL) -1 beta, tumour necrosis factor (TNF) -alpha, IL-6, neutrophil elastase and interferon (IFN) -gamma. Results Anti-elafin antibody showed a strong reaction with the subcorneal region of the epidermis in patients with Behcet's syndrome, Sweet's syndrome, pyoderma gangrenosum, cutaneous allergic vasculitis and acute bacterial infection (cellulitis), but showed no reaction in skin from patients with dermal lymphocyte infiltration such as is seen in chronic prurigo and discoid lupus erythematosus. The in vitro experiments demonstrated that treatment with IL-1 beta and TNF-alpha resulted in 2.6-fold and 4-fold stimulation of elafin secretion, respectively, whereas IL-6, neutrophil elastase and IFN-gamma caused no significant changes in elafin release. Conclusions These results suggest that inflammatory mediators such as IL-1 beta or TNF-alpha secreted by dermal neutrophils may be involved in overexpression of elafin in keratinocytes; this could protect the epidermis from degradation by dermal neutrophil infiltration.
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