Journal
MOLECULAR PLANT-MICROBE INTERACTIONS
Volume 13, Issue 10, Pages 1156-1160Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI.2000.13.10.1156
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Uricase (nodulin-35) cDNA, LjUr, was isolated from nodules of a model legume, Lotus japonicus. LjUr expression was most abundant in nodules, although it was detected in nonsymbiotic tissues as well, particularly in roots. Expression in nodules was detected in uninfected cells, nodule parenchyma, and, more intensely, in vascular bundles. Phylogenetic analysis of uricase sequences from various legumes indicated that uricases of amide- and ureide-transporting legumes form two distinct clades. LjUr is in the cluster of amide-transport legumes even though L. japonicus bears determinate nodules.
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