Journal
PEPTIDES
Volume 21, Issue 10, Pages 1511-1517Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0196-9781(00)00305-3
Keywords
proglucagon-derived peptides; glucagon-like peptide 2; metabolism; autoradiography; rat
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Glucagon-like peptide 2 (GLP-2) is a 33-amino acid (1-33) intestinotrophic peptide. In this study, the distribution and binding of i.v. injected radiolabeled GLP-2 (1-33) were investigated in rats using autoradiography in order to target possible binding sites. The major part of I-125-GLP-2 (1-33) was distributed to kidneys, liver, and the gastrointestinal tract. In the small intestine, a high density of grains was localized in the epithelium with a predominance in the luminal part of the villus. The saturability of I-125-GLP-2 (1-33) was investigated by administration of excess amounts of non-radioactive GLP-2 (1-33) or the primary metabolite of GLP-2 degradation, GLP-2 (3-33). In the small intestine, I-125-GLP-2 was displaced both by non-radioactive GLP-2 (1-33) acid (3-33), suggesting that the uptake of GLP-2 (1-33) in the small intestine is receptor-specific and that the metabolite GLP-2 (3-33) may interact with the GLP-2 receptor. (C) 2000 Elsevier Science Inc. All rights reserved.
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