Journal
BIOLOGIA PLANTARUM
Volume 52, Issue 1, Pages 1-8Publisher
SPRINGER
DOI: 10.1007/s10535-008-0001-z
Keywords
Dianthus caryophyllus; in vitro culture; leaf parenchyma
Categories
Ask authors/readers for more resources
Carnation plantlets (Dianthus caryophyllus L.) cultured in vitro often develop morphological and physiological anomalies, a phenomenon called hyperhydricity, which impairs their survival ex vitro. When the agar concentration of the growth medium was increased (from 0 to 12 g dm(-3)), thereby reducing water availability, the hyperhydricity of those adventitious shoots regenerated from carnation petals decreased. This was accompanied by a progressive fall in the water content of shoots (94.9 to 91.4 %), fresh mass (from 57.2 to 1.8 mg), number of leaf parenchyma cell layers (from 9.3 to 7.7), and the size of these cells (from 968 to 254 mu m(2)). However, the number of regenerated shoots also decreased (17.7 in 2 g dm(-3) agar to 4.3 in 12 g dm(-3)). Similarly, in ventilated tubes, which exhibit a lower relative humidity than tightly closed tubes, shoot organogenesis diminished up to 28 %, in tandem with shoot water content. Thus, relative humidity and water availability in culture vessels do not only influence shoot hyperhydricity in carnations, but also greatly affect adventitious shoot organogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available