Journal
BIOINTERPHASES
Volume 7, Issue 1-4, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1007/s13758-012-0065-3
Keywords
-
Categories
Funding
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/61390/2009, SFRH/BPD/45206/2008]
- International Max-Planck-Research School
- EU [NMP4-SL-2009-229292]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/61390/2009] Funding Source: FCT
Ask authors/readers for more resources
Bioactive constructs to guide cellular mobilization and function have been proposed as an approach for a new generation of biomaterials in functional tissue engineering. Adult mesenchymal stem cells have been widely used as a source for cell based therapeutic strategies, namely tissue engineering. This is a heterogeneous cell population containing many subpopulations with distinct regenerative capacity. Thus, one of the issues for the effective clinical use of stem cells in tissue engineering is the isolation of a highly purified, expandable specific subpopulation of stem cells. Antibody functionalized biomaterials could be promising candidates to isolate and recruit specific cell types. Here we propose a new concept of instructive biomaterials that are able to recruit and purify specific cell types from a mixed cell population. This biomimetic concept uses a target-specific chitosan substrate to capture specific adipose derived stem cells. Specific antibodies were covalently immobilized onto chitosan membranes using bis[sulfosuccinimidyl] suberate (BS3). Quartz crystal microbalance (QCM) was used to monitor antibody immobilization/adsorption onto the chitosan films. Specific antibodies covalently immobilized, kept their bioactivity and captured specific cell types from a mixed cell population. Microcontact printing allowed to covalently immobilize antibodies in patterns and simultaneously a spatial control in cell attachment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available