Journal
ANIMAL REPRODUCTION SCIENCE
Volume 63, Issue 1-2, Pages 101-111Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4320(00)00150-0
Keywords
pig ovarium; oocyte activation; calcium ionophore; cycloheximide
Ask authors/readers for more resources
In vitro matured pig oocytes were activated using a combined treatment of calcium ionophore A 23187 with cycloheximide. The oocytes were exposed to ionophore (10, 25 or 50 mu M) for 0.5, 1, 3, 5 or 7 min and then cultured with cycloheximide (0 or 10 mu g/ml) for 6 h. Cycloheximide treatment significantly increased the activation rate of oocytes and the percentage of oocytes that were able to develop after activation. The highest activation rate was observed after treatment with 50 mu M ionophore. The highest percentage of developing eggs was observed after combined treatment of ionophore (25 mu M) with cycloheximide. The percentage of oocytes developing up to the morula and blastocyst stage was not significantly increased after cycloheximide treatment. (C) 2000 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available