Journal
BIOINTERPHASES
Volume 5, Issue 3, Pages FA41-FA47Publisher
AMER INST PHYSICS
DOI: 10.1116/1.3474475
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Funding
- Bruker Optik Southeast Asia
- IMRE
- Provost's Office and School of Materials Science and Engineering of Nanyang Technological University
- Analytical Technologies Pte Ltd.
- National University of Singapore [WBS-R143-000-374-112]
- Singapore National Research Foundation [NRF2009-POC001-04]
- Deutsche Forschungsgemeinschaft (DFG) [SFB 625]
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Whole cells are attractive biocatalysts, particularly if the reaction requires cofactors or involves multiple transformations. Immobilization of the catalyst is often a prerequisite for continuous processes. The highly cationic chemically modified plasma protein bovine serum albumin (cBSA-147) has been applied for the electrostatically mediated immobilization of the planktonic bacterium E. coli BL21 star (DE3), and the resulting biofilms were superior to those formed on poly-L-lysine coated surfaces. The biocatalyst was immobilized in a capillary column (inside diameter of 530 mu m and L=30 m) and evaluated in the enantioselective reduction of ethyl acetoacetate to R-(-)ethyl hydroxybutyrate. In continuous operation in the microreactor format, the productivity of the cells was about 30% higher than that determined in a bench-scale fermentation system. This increase is attributed to the improved mass transfer over short geometrical dimensions. The similarity in the results indicates that studies on a biofilm-coated microreactor can be used for the accelerated collection of data for process optimization. (C) 2010 American Vacuum Society. [DOI: 10.1116/1.3474475]
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