Journal
FEBS LETTERS
Volume 482, Issue 3, Pages 257-260Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02070-6
Keywords
cellular prion protein; laminin; extracellular matrix; neurite outgrowth; PC-12 cell; cell differentiation
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Prions, the etiological agents for infectious degenerative encephalopathies, act by inducing structural modifications in the cellular prion protein (PrPc). Recently, we demonstrated that PrPc binds laminin (LN) and that this interaction is important for the neuritogenesis of cultured hippocampal neurons. Here we have used the PC-12 cell model to explore the biological role of LN-PrPc interaction. Antibodies against PrPc inhibit cell adhesion to LN-coated culture plaques. Furthermore, chromophore-assisted laser inactivation of cell surface PrPc perturbs LN-induced differentiation and promotes retraction of mature neurites. These results point out to the importance of PrPc as a cell surface ligand for LN. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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