Journal
FEBS LETTERS
Volume 483, Issue 1, Pages 57-61Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)02084-6
Keywords
tumor necrosis factor alpha; mitogen-activated protein kinase p38; mRNA stability; translation; macrophage; lipopolysaccharide
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The translation of tumour necrosis factor alpha (TNF alpha) mRNA is regulated by the stress-activated protein kinase p38, which also controls the stability of several pro-inflammatory mRNAs. The regulation of TNF alpha gene expression in a mouse macrophage cell line RAW264.7 was re-examined using an inhibitor of stress-activated protein kinases. Stimulation of these cells with bacterial lipopolysaccharide resulted in stabilisation of TNF alpha mRNA, which was reversed by specific inhibition of p38, An adenosine/uridine-rich element from the TNF alpha 3' untranslated region conferred p38-sensitive decay in a tetracycline-regulated mRNA stability assay. Therefore the p38 pathway also controls TNF alpha mRNA turnover. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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