Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 277, Issue 1, Pages 72-78Publisher
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2000.3634
Keywords
hepatic stellate cells; collagen gel contraction; lysoposphatidic acid; Rho-kinase; focal adhesion; actomyosin-interaction; electric cell-substrate imedance sensor
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Funding
- NIAAA NIH HHS [R01 AA09231] Funding Source: Medline
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We studied the effect of lysophosphatidic acid (LPA) on collagen gel contraction by cultured rat hepatic stellate cells (HSCs) in association with the function of Rho-kinase, one of the target molecules of small GTPase Rho, Binding studies showed a single class-binding site of LPA on HSCs. LPA enhanced the contraction of a collagen lattice seeded with HSCs, LPA increased the number of HSCs with polygonal morphology that contained actin stress fibers, and enhanced the phosphorylation of myosin light chain and the assembly of focal adhesion kinase and RhoA around fibronectin-coated beads seeded on HSCs. The electric cell-substrate impedance sensor system showed that LPA enhanced adhesion of HSC to extracellular substrate. All the effects of LPA were suppressed by Y-27632, Rho-kinase inhibitor. These data support the notion that LPA is involved in modulating HSC morphology, its attachment to surrounding extracellular matrix and its contraction by a mechanism involving Rho-kinase. (C) 2000 Academic Press.
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