Journal
ANALYTICAL BIOCHEMISTRY
Volume 285, Issue 2, Pages 246-254Publisher
ACADEMIC PRESS INC
DOI: 10.1006/abio.2000.4783
Keywords
high-performance liquid chromatography; cardiolipin; mass spectrometry
Funding
- NIA NIH HHS [1RO1AG12447, 1K04AG00676] Funding Source: Medline
- NIDDK NIH HHS [1RO1DK36069] Funding Source: Medline
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We describe a comprehensive approach to the separation, quantitation, and characterization of phospholipids and lysophospholipids present in complex biological samples. The central feature is a normal-phase HPLC separation of individual phospholipid and lysophospholipid classes. In this single chromatographic step, phospholipids and lysophospholipids are separated and recovered for quantitation by organic phosphate assay and characterization by acyl-group composition. Recovery of phospholipids and lysophospholipids from HPLC averages 80-90%. Isolated phospholipid and lysophospholipid fractions are available for separation of individual molecular species by second-dimension reverse-phase HPLC and characterization of individual molecular species by mass spectrometry. (C) 2000 Academic Press.
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