Journal
EMBO JOURNAL
Volume 19, Issue 20, Pages 5552-5561Publisher
OXFORD UNIV PRESS
DOI: 10.1093/emboj/19.20.5552
Keywords
ERCC1; gene targeting; homologous recombination; terminal non-homology; XpF-Ercc1 endonuclease
Categories
Funding
- NCI NIH HHS [CA28711, CA36361] Funding Source: Medline
- NIEHS NIH HHS [P30 ES007784] Funding Source: Medline
- NIGMS NIH HHS [R01 GM038219, GM38219] Funding Source: Medline
Ask authors/readers for more resources
The XpF/Ercc1 structure-specific endonuclease performs-the 5' incision in nucleotide excision repair and is the apparent mammalian counterpart of the Rad1/Rad10 endonuclease from Saccharomyces cerevisiae. In yeast, Rad1/Rad10 endonuclease also functions in mitotic recombination, To determine whether XpF/Ercc1 endonuclease has a similar role in mitotic recombination, we targeted the APRT locus in Chinese hamster ovary ERCC1(+) and ERCC1(-) cell lines with insertion vectors having long or short terminal non-homologies flanking each side of a double-strand break, No substantial differences were evident in overall recombination frequencies, in contrast to results from targeting experiments in yeast, However, profound differences were observed in types of APRT(+) recombinants recovered from ERCC1(-) cells using targeting vectors with long terminal non-homologies-almost complete ablation of gap repair and single-reciprocal exchange events, and generation of a new class of aberrant insertion/deletion recombinants absent in ERCC1(+) cells. These results represent the first demonstration of a requirement for ERCC1 in targeted homologous recombination in mammalian cells, specifically in removal of long non-homologous tails from invading homologous strands.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available