Journal
BIOINFORMATICS
Volume 30, Issue 8, Pages 1180-1182Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btt764
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Funding
- National Natural Science Foundation of China (NSFC) [31110103904]
- 973 programs [2011CBA01000, 2012CB945101, 2011CBA01102]
- NSFC [31171242]
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The CRISPR/Cas or Cas9/guide RNA system is a newly developed, easily engineered and highly effective tool for gene targeting; it has considerable off-target effects in cultured human cells and in several organisms. However, the Cas9/guide RNA target site is too short for existing alignment tools to exhaustively and effectively identify potential off-target sites. CasOT is a local tool designed to find potential off-target sites in any given genome or user-provided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches allowed in the seed and non-seed regions.
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