Differential inhibitory effects of sophoricoside analogs on bioactivity of several cytokines

Effects of sophoricoside and its analogs on proinflammatory cytokines have been investigated. Sophoricoside, genistein and orobol exhibited inhibitory effects on IL-5, IL-3, GM-CSF and IL-6 bioactivities, Genistin showed inhibitory effects on IL-5 and IL-3 bioactivities, but did not inhibit GMCSF and IL-6 bioactivities. None of the sophoricoside analogs showed inhibitory effects on both IL-1 beta and TNF-alpha bioactivities. Among the compounds, sophoricoside exhibited the highest inhibitory effects on IL-5, IL-3 and IL-6 bioactivities with IC50 values of 1.9 muM, 6.9 muM and 6.0 muM, respectively and orobol did show on GM-CSF bioactivity with an IC50 value of 18.0 muM. The result would provide an additional mechanism by which the compounds exert immunosuppressive and anti-inflammatory effects. (C) 2000 Elsevier Science Inc. All rights reserved.