Journal
JOURNAL OF CELL BIOLOGY
Volume 151, Issue 3, Pages 639-651Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.151.3.639
Keywords
chromosome condensation; Drosophila melanogaster; myoblast fusion; sarcomere; titin
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Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin, and show that it encodes a two-megadalton protein with significant colinear homology to the NH2-terminal half of vertebrate titin. Mutations in D-Titin cause chromosome undercondensation, chromosome breakage, loss of diploidy, and premature sister chromatid separation. Additionally, D-Titin mutants have defects in myoblast fusion and muscle organization. The phenotypes of the D-Titin mutants suggest parallel roles for titin in both muscle and chromosome structure and elasticity, and provide new insight into chromosome structure.
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