Journal
BIOINFORMATICS
Volume 30, Issue 4, Pages 566-568Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btt702
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Funding
- BBSRC National Capability Grant [BB/J010375/1]
- EU Framework 7 Programme (TransPLANT) [283496]
- BBSRC [BBS/E/T/000PR5885, BBS/E/T/000PR6193, BB/M004805/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/T/000PR5885, BBS/E/T/000PR6193, BB/M004805/1] Funding Source: researchfish
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Illumina's recently released Nextera Long Mate Pair (LMP) kit enables production of jumping libraries of up to 12 kb. The LMP libraries are an invaluable resource for carrying out complex assemblies and other downstream bioinformatics analyses such as the characterization of structural variants. However, LMP libraries are intrinsically noisy and to maximize their value, post-sequencing data analysis is required. Standardizing laboratory protocols and the selection of sequenced reads for downstream analysis are non-trivial tasks. NextClip is a tool for analyzing reads from LMP libraries, generating a comprehensive quality report and extracting good quality trimmed and deduplicated reads.
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