Journal
EMBO JOURNAL
Volume 19, Issue 21, Pages 5824-5834Publisher
WILEY
DOI: 10.1093/emboj/19.21.5824
Keywords
HPRI; THO2; MFT1; THP2; mitotic recombination; THO protein complex; transcription-associated recombination transcription elongation
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Funding
- NCI NIH HHS [P30 CA08748, P30 CA008748] Funding Source: Medline
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Transcription-induced recombination has been reported in all organisms from bacteria to mammals. We have shown previously that the yeast genes HPR1 and THO2 may be keys to the understanding of transcription-associated recombination, as they both affect transcription elongation and hyper-recombination in a concerted manner. Using a yeast strain that has the wild-type THO2 gene replaced by one encoding a His(6)-HA-tagged version, we have isolated an oligomeric complex containing four proteins: Tho2, Hpr1, Mft1 and a novel protein that we have named Thp2, We have reciprocally identified a complex containing Hpr1, Tho2 and Mft1 using anti-Mft1 antibodies in immunoprecipitation experiments. The protein complex is mainly nuclear; therefore, Tho2 and Hpr1 are physically associated, Like hpr1 Delta and tho2 Delta cells, mft1 Delta and thp2 Delta cells show mitotic hyper-recombination and impaired transcription elongation, in particular, through the bacterial lacZ sequence. Hyper-recombination conferred by mft1 Delta and thp2 Delta is only observed in DNA regions under transcription conditions. We propose that this protein complex acts as a functional unit connecting transcription elongation with the incidence of mitotic recombination.
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