New lignan glycosides with potent antiinflammatory effect, isolated from Justicia ciliata

Two new Lignan glycosides, 4-O-[alpha -L-arabinopyranosyl-(1'-->2)-beta -D-xylopyranosyl-1'''-->5)-beta -D-apiofura- nosyl]diphyllin (1), named ciliatoside A (1), and 4-O-({[beta -D-apiofuranosyl-(1'-->3')-alpha -L-arabinopyranosyl-(1'-->2)] [beta -D-xylopyranosyl-(1'-->5)]-beta -D-apiofuranosyl}diphyllin (2), named ciliatoside B (2), were isolated from the whole plant of Justicia ciliata. The structures of 1 and 2 were determined by spectral and chemical methods. Compounds 1 and 2 strongly inhibited the accumulation of NO2- in lpopolysaccharide-stimulated RAW 264.7 cells in a concentration-dependent manner with IC50 values of 27.1 +/- 1.6 and 29.4 +/- 1.4 muM, respectively.