Journal
ANALYTICAL CHEMISTRY
Volume 72, Issue 21, Pages 5300-5307Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0003798
Keywords
-
Categories
Ask authors/readers for more resources
We report here a novel bioelectrode based on self-assembled multilayers of polyphenol oxidase intercalated with cationic polyallylamine built up on a thiol-modified field surface. We use an immobilization strategy previously described by Hodak J, et al. (Langmuir 1997, 13, 2708-2716) Quartz crystal microbalance with electro-acustic impedance experiments were carried out to follow quantitatively the multilayer film formation. The response of the self-assembly polyphenol oxidase-polyallylamine electrodes toward different metabolically related cate-cholamines was studied, to evaluate enzyme kinetics. For the analyzed compounds, only dopamine and its metabolite Dopac gave catalytic currents at applied potential close to 0V. These responses were proportional to the number of polyphenol oxidase-immobilized layers and were also controlled by the enzymatic reaction. The combination of microgravimetric and electrochemical techniques allowed us to determine the kinetic enzymatic constants, showing that the decomposition rate for the enzyme-substrate complex is slower than the enzymatic reoxidation step.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available