Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 20, Issue 2, Pages 252-264Publisher
ACADEMIC PRESS INC
DOI: 10.1006/prep.2000.1286
Keywords
codon usage; eukaryotic signal peptide; Fab '; mRNA structure; E. coli
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We investigated the ability of signal peptides of eukaryotic origin (human, mouse, and yeast) to efficiently direct model proteins to the Escherichia coli periplasm. These were compared against a well-characterized prokaryotic signal peptide-OmpA. Surprisingly, eukaryotic signal peptides can work very efficiently in E. coli, but require optimization of codon usage by codon-based mutagenesis of the signal peptide coding region. Analysis of the 5' of periplasmic and cytoplasmic E. coli genes shows some codon usage differences. (C) 2000 Academic Press.
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