Journal
BIOINFORMATICS
Volume 27, Issue 24, Pages 3432-3434Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btr582
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Funding
- National Basic Research Project (973 program) [2012CB518200]
- National Natural Science Foundation of China [81170558, 81070741, 30900862]
- State Key Laboratory of Proteomics of China [SKLP-Y200906, SKLP-K201004, SKLP-O201002]
- Special Key Program for Science and Technology of China [2012ZX09102301-016]
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The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure and JavaScript to interact with the users. In VizPrimer, the users can focus their attention on the gene structure and primer design strategy, without wasting time calculating the exon positions of splice variants or manually configuring complicated parameters. In addition, VizPrimer is also suitable for the design of PCR primers for amplifying open reading frames and detecting single nucleotide polymorphisms (SNPs).
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