Journal
HORMONE AND METABOLIC RESEARCH
Volume 32, Issue 11-12, Pages 548-554Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/s-2007-978684
Keywords
ionomycin; dibutyryl-cycloAMP; Tumour Necrosis Factor alpha (TNFalpha) apM1-antibody; Acrp30/AdipoQ; GBP28; adiponectin
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3T3-L1-adipocytes produce the adipocyte complement related protein of 30 kD (Acrp30), which is also designated as AdipoQ, In order to study the expression and secretion of the human homologue of this protein, apM1 (adipose Most abundant gene transcript I, also named gelatin-binding protein of 28 kD [GBP28] or adiponectin), a polyclonal antibody was produced. Both expression and secretion can be detected beginning with day 4 after induction of differentiation. The amount of expressed apM1 correlates with the specific activity of the differentiation marker glycerol-3-phosphate dehydrogenase. Secretion of apM1 is increased by the addition of ionomycin. Both the nonhydrolysable dibutyryl-cycloAMP and tumour necrosis factor alpha reduce the expression and secretion of apM1.
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