Journal
BIOINFORMATICS
Volume 27, Issue 2, Pages 266-267Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btq624
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Funding
- National Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human Services [HHSN266200400001C]
- Bill & Melinda Gates Foundation
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PriSM is a set of algorithms designed to select and match degenerate primer pairs for the amplification of viral genomes. The design of panels of hundreds of primer pairs takes just hours using this program, compared with days using a manual approach. PriSM allows for rapid in silico optimization of primers for downstream applications such as sequencing. As a validation, PriSM was used to create an amplification primer panel for human immunodeficiency virus (HIV) Clade B.
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