Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 64, Issue 11, Pages 2497-2499Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.64.2497
Keywords
Geotrichum; lipase; positional specificity; stereoselectivity; tributyrin
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The extracellular Lipases A and C produced by Geotrichum sp. FO401B have a preference for the sn-1,3 and sn-2 positions of triglyceride, respectively. Total production of these lipases was increased by plant oils and tributyrin. Butyl Toyopearl column chromatography demonstrated that only Lipase C was produced in the presence of tributyrin. Lipase C hydrolysed natural fats except sardine oil preferentially at the sn-2 position, but it showed little stereoselectivity for triolein.
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