Journal
BIOINFORMATICS
Volume 24, Issue 20, Pages 2339-2343Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btn450
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Funding
- DOE BACTER [DE-FG2-04ER25627]
- Center for Eukaryotic Structural Genomics NIH/NIGMS [U54 GM074901-01, P50 GM064598]
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Motivation: For many biotechnological purposes, it is desirable to redesign proteins to be more structurally and functionally stable at higher temperatures. For example, chemical reactions are intrinsically faster at higher temperatures, so using enzymes that are stable at higher temperatures would lead to more efficient industrial processes. We describe an innovative and computationally efficient method called Improved Configurational Entropy (ICE), which can be used to redesign a protein to be more thermally stable (i.e. stable at high temperatures). This can be accomplished by systematically modifying the amino acid sequence via local structural entropy (LSE) minimization. The minimization problem is modeled as a shortest path problem in an acyclic graph with nonnegative weights and is solved efficiently using Dijkstras method.
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