Journal
MOLECULAR CELL
Volume 6, Issue 5, Pages 1077-1087Publisher
CELL PRESS
DOI: 10.1016/S1097-2765(00)00106-4
Keywords
-
Categories
Funding
- NIGMS NIH HHS [R01 GM037706, GM07321, GM37706, GM58800] Funding Source: Medline
Ask authors/readers for more resources
In RNA-mediated interference (RNAi), externally provided mixtures of sense and antisense RNA trigger concerted degradation of homologous cellular RNAs. We show that RNAi requires duplex formation between the two trigger strands, that the duplex must include a region of identity between trigger and target RNAs, and that duplexes as short as 26 bp can trigger RNAi. Consistent with in vitro observations, a fraction of input dsRNA is converted in vivo to short segments of similar to 25 nt. Interference assays with modified dsRNAs indicate precise chemical requirements for both bases and backbone of the RNA trigger. Strikingly, certain modifications are well tolerated on the sense, but not the antisense, strand, indicating that the two trigger strands have distinct roles in the interference process.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available