Journal
VETERINARY PARASITOLOGY
Volume 93, Issue 1, Pages 39-46Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-4017(00)00326-5
Keywords
Fasciola hepatica; cattle-trematoda; indirect-ELISA; sandwich-ELISA; diagnosis-trematoda
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An indirect-enzyme immune-linked immunosorbent assay (IEA) was compared to a sandwich-enzyme-linked immunosorbent assay (SEA) to diagnose fasciolosis in naturally infected cattle. By means of a rabbit polyclonal IgG antibody to F. hepatica antigens, captured circulating antigens were detected by an SEA technique. A total of 85.1% of the samples examined was considered positive by using the IEA-test, whereas this percentage was of 37.3% by the SEA test. Using the two enzymatic probes, only the 38.3% resulted positive to both immunoassay probes. Our data indicate that circulating antigen detection assay in combination with results of antibody detection may offer a more reliable diagnostic technique than classic coprologic ones, with results very appropriate for epidemiologic studies carried out on a wide area. (C) 2000 Elsevier Science B.V. All rights reserved.
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