Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 56, Issue 12, Pages 2491-2497Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S1386-1425(00)00340-1
Keywords
Morin; DNA; ZnL2 center dot 3H(2)O; intercalation; binding
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The interactions of Morin (2', 3, 4', 5, 7-pentahydroxyflavone) and its Zn-complex, ZnL2. 3H(2)O [L = Morin (2'-OH group deprotonated)], with calf thymus DNA have been studied using fluorimetric and electrochemical methods. ZnL2. 3H(2)O has different spectral characteristics and electrochemical behavior from that of Morin in the presence of DNA. Increasing fluorescence is seen for ZnL2. 3H(2)O with DNA addition whereas decreased fluorescence is observed for Morin. An isosbestic point appears at 560 nm for the DNA-ZnL2. 3H(2)O system with ethidium bromide (EB) addition while no isosbestic point is detectable for Morin. Quenching fluorescence is observed for DNA-EB system when ZnL2. 3H(2)O is added whereas no quenched fluorescence is seen for DNA-EB system with Morin addition. The above results suggest that Morin and ZnL2. 3H(2)O can both bind to DNA, but the binding mode is different. The complex binds to DNA mainly by intercalation, while Morin binds in a non-intercalating mode. The binding constant and binding site sizes are derived from electrochemical methods. For ZnL2. 3H(2)O, the binding constant is 5.0 x 10(4) 1 mol(-1) at 20 degrees C, and the binding site size n(s) is 5. (C) 2000 Elsevier Science B.V. All rights reserved.
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