Journal
FEMS MICROBIOLOGY LETTERS
Volume 192, Issue 1, Pages 33-38Publisher
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6968.2000.tb09355.x
Keywords
Bacillus subtilis; YabG protein; spore coat protein; protease
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The synthesis and proteolysis of the spore coat proteins, SpoIVA and YrbA, of Bacillus subtilis were analyzed using antisera. Almost no intact full-length proteins of either type were extracted from wild-type spores, while yabG mutant spores contained intact SpoIVA and YrbA proteins. We purified recombinant YrbA and YabG proteins from Escherichia coli transformants and found that YrbA was cleaved to the smaller moiety in the presence of YabG in vitro. These observations indicate that YabG is a protease involved in the proteolysis and maturation of SpoIVA and YrbA proteins, conserved with the cortex and/or coat assembly by B. subtilis. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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