Journal
JOURNAL OF BIOCHEMISTRY
Volume 128, Issue 5, Pages 723-725Publisher
JAPANESE BIOCHEMICAL SOC
DOI: 10.1093/oxfordjournals.jbchem.a022807
Keywords
hydrolase; nucleoporins; Smt3; SUMO-1; Ulp1
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Yeast Smt3 is a ubiquitin-like protein similar to the mammalian SUMO-1. Cdc3, a septin component, is known to be modified by Smt3, The level of this modification was affected by Smt3-specific protease mutation ulp1-ts or overexpression of ULP1. By two-hybrid screening, we isolated 5 UIP (Ulp1 Interacting protein) genes. UIP1 was identical to NUP42 encoding a component of the nuclear pore complex (NPC), Gle1, another NPC-associating component, also interacted with Ulp1 in the two-hybrid system and coimmunoprecipitation experiment. Thus Ulp1 associates with nucleoporins and may interact with septin rings in the telophase.
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