Journal
ANESTHESIOLOGY
Volume 93, Issue 5, Pages 1285-1293Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00000542-200011000-00023
Keywords
global cerebral ischemia; neuronal calcium; resuscitation; volatile anesthetic
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Funding
- NIGMS NIH HHS [R01 GM50686] Funding Source: Medline
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Background Inhalational anesthetics are neuroprotective in rat models of global ischemia, To determine whether isoflurane at a clinically relevant concentration is neuroprotective In a canine model of cardiac arrest, we measured neurologic function and hippocampal Ca2+/calmodulin-dependent protein kinase II (CaMKII) content 20 h after cardiac arrest. Methods: We tested the neuroprotective effect of 30 min of 1.5% isoflurane exposure before 8 min of global ischemia induced with ventricular fibrillation. Animals were randomized to four groups: control, isoflurane-control, ischemia, and isoflurane-ischemia. After resuscitation and 20 h of Intensive care, each animal's neurologic deficit score was determined by two blinded evaluators. The hippocampal content of CaMKII, determined by immunoblotting, was measured by an Individual blinded to the treatment groups. CaMKII activity was measured in samples from the cortex, hippocampus, and striatum of animals in each group. Results: Isoflurane-ischemic animals had a median neurologic deficit score of 22.6% compared with 43.8% for the ischemic animals (P < 0.05) Hippocampal levels of the -subunit of CaMKII (CaMKII beta) were relatively preserved in isoflurane-ischemic animals (68 +/- 4% of control) compared with ischemic animals (48 +/- 2% of control; P < 0.001), although both groups were statistically significantly lower than control (P < 0.001 ischemia as. control and P < 0.05 isoflurane-ischemia Ds. control), Conclusions: Isoflurane Is an effective neuroprotective drug in a canine cardiac arrest model in terms of both functional and biochemical criteria.
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