Journal
ALLERGY
Volume 55, Issue 11, Pages 1011-1018Publisher
WILEY
DOI: 10.1034/j.1398-9995.2000.00685.x
Keywords
atopic dermatitis; CD30; human Th1/Th2 cells; interferon-gamma; interleukin-4
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Th2 clones have been reported to express CD30 preferentially, but whether T cells producing Th2-type cytokines may favor CD30 expression in the in who state remains unknown. We investigated the expression of CD30 on circulating T cells in atopic dermatitis (AD) as a Th2-dominated disorder. Peripheral blood mononuclear cells were prepared from 51 AD patients and 14 nonatopic controls, and their phenotypes were analyzed with flow cytometry. Cytokine production by stimulated CD4(+) T cells was also assessed by the single-cell-staining method. Flow cytometric analysis clearly revealed that CD30(+) T cells were identifiable in the blood of AD patients with greater frequency compared to controls. The important finding was that CD30 expression was restricted to a small but substantial population of memory (CD45RO(+)) CD4(+) T cells, but not CD8(+) ones. In AD patients, it was demonstrated that the percentages of CD30(+) cells within CD45RO(+) CD4(+) T cells correlated well with the disease severity, serum IgE levels, peripheral eosinophil counts, and tendency toward Th2-dominant cytokine pattern as determined by the ratio of interleukin-4 to interferon-gamma production. This study suggests that CD30 expression in circulating T cells might serve as an in vivo marker for the Th2-dominated condition.
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