Journal
BIOFOULING
Volume 30, Issue 6, Pages 667-674Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2014.905927
Keywords
Staphylococcus aureus; methicillin-resistant Staphylococcus aureus (MRSA); chimeric lysin; biofilm; anti-biofilm; susceptibility
Funding
- Ministry of Science and Technology of China [2012CB721102]
- National Natural Science Foundation of China [21105114]
- Key Laboratory on Emerging Infectious Diseases and Biosafety in Wuhan
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Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for a large number of chronic infections due to its ability to form robust biofilms. Herein, the authors evaluated the anti-biofilm activity of a Staphylococcus specific chimeric lysin ClyH on MRSA biofilms. ClyH is known to be active against planktonic MRSA cells in vitro and in vivo. The minimum concentrations for biofilm eradication (MCBE) of ClyH were 6.2-50 mg l(-1), much lower than those of antibiotics. Scanning electron microscope (SEM) analysis revealed that ClyH eliminated MRSA biofilms through cell lytic activity in a time-dependent manner. Viable plate counts and kinetic analysis demonstrated that biofilms of different ages displayed varying susceptibility to ClyH. Together with previously demonstrated in vivo efficacy of ClyH against MRSA, the degradation efficacy against biofilms of different ages indicates that ClyH could be used to remove MRSA biofilms in vivo.
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