Journal
BIOFOULING
Volume 29, Issue 10, Pages 1253-1268Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2013.835805
Keywords
Listeria monocytogenes; desiccation tolerance; mixed species biofilms; spoilage bacteria; Jameson effect; scanning electron microscopy
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Foundation for Innovation
- Atlantic Innovation Fund
- NSERC
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This study investigated the dynamics of static biofilm formation (100% RH, 15 degrees C, 48-72h) and desiccation survival (43% RH, 15 degrees C, 21days) of Listeria monocytogenes, in dual species biofilms with the common spoilage bacteria, Pseudomonas fluorescens, Serratia proteamaculans and Shewanella baltica, on the surface of food grade stainless steel. The Gram-negative bacteria reduced the maximum biofilm population of L. monocytogenes in dual species biofilms and increased its inactivation during desiccation. However, due to the higher desiccation resistance of Listeria relative to P. fluorescens and S. baltica, the pathogen survived in greater final numbers. In contrast, S. proteamaculans outcompeted the pathogen during the biofilm formation and exhibited similar desiccation survival, causing the N-21 days of Serratia to be ca 3 Log(10)(CFUcm(-2)) greater than that of Listeria in the dual species biofilm. Microscopy revealed biofilm morphologies with variable amounts of exopolymeric substance and the presence of separate microcolonies. Under these simulated food plant conditions, the fate of L. monocytogenes during formation of mixed biofilms and desiccation depended on the implicit characteristics of the co-cultured bacterium.
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