Journal
JOURNAL OF VIROLOGY
Volume 74, Issue 22, Pages 10816-10818Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.22.10816-10818.2000
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Funding
- NCI NIH HHS [R01 CA082396, CA82396] Funding Source: Medline
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A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.
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