4.6 Article

Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts

Journal

JOURNAL OF VIROLOGY
Volume 74, Issue 22, Pages 10816-10818

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.22.10816-10818.2000

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Funding

  1. NCI NIH HHS [R01 CA082396, CA82396] Funding Source: Medline

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A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

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